CAM Conference

Nutrition and Cancer

Dietary Isothiocyanates Inhibit the Growth of Human Bladder Carcinoma Cells1

Li Tang and Yuesheng Zhang2

Department of Chemoprevention, Roswell Park Cancer Institute, Buffalo, NY 14263

2To whom correspondence should be addressed. E-mail:

Many isothiocyanates (ITCs), some of which are abundant in cruciferous vegetables, have been repeatedly shown to inhibit carcinogenesis in a variety of rodent organs.

However, several naturally occurring ITCs also promoted bladder tumorigenesis in rodents, raising the question of whether ITCs behave differently in bladder cells.

Alternatively, the observed carcinogenic effects of ITCs may result from prolonged exposure of the bladder epithelium, where the tumors originate, to high concentrations of electrophilic ITCs in the urine.

Ingested ITCs are almost exclusively excreted and highly concentrated in the urine as N-acetylcysteine conjugates (NAC-ITC). While several NAC-ITCs also are known anticarcinogens, they are unstable and readily dissociate into parent ITCs.

In this study, ITCs, including those that have carcinogenic potential in the rodent bladders, induced apoptosis and/or arrested cell-cycle progression in 2 human bladder carcinoma lines (UM-UC-3 and T24) at 7.5–30 µmol/L. Multiple caspases, including caspase-9, -8, and -3, as well as poly(ADP-ribose)polymerase, were cleaved upon ITC exposure.

The ITCs blocked cell-cycle progression at the G2/M and/or S phases in these cells and downregulated several cell-cycle regulators. However, further increases in ITC concentrations abolished their activities, described above.

These findings show that urinary ITC concentrations may need to be maintained at low micromolar concentrations for bladder cancer prevention.

J. Nutr. 134:2004-2010, August 2004

Annie Appleseed Project - AAPR bottom logo

AAPR | Help us today!

Donate to AAPR

Get Updates by Email

Remember: We are NOT Doctors and have NO medical training. DISCLAIMER: The information provided is for educational purposes only. It is not meant to diagnose or treat any health condition and is not a replacement for treatment by a healthcare provider. This article or site may contain copyrighted material, the use of which has not always been specifically authorized by the copyright owner. We are making such material available in our efforts to advance understanding of health, political, human rights, economic, democratic, and social justice issues, etc. We believe this constitutes a "fair use" of any such copyrighted material as provided for in section 107 of the US Copyright Law. In accordance with Title 17 U.S.C. Section 107, this material is distributed without profit to those who have expressed an interest in receiving the included information for research and educational purposes. For more information go to: Cornell University Law School.

Annie Appleseed Project - certified by Health On the Net Foundation This site complies with the HONcode standard for trustworthy health information: verify here.

Search only trustworthy HONcode health websites: