CAM ConferenceAntrodia camphorata extract induces replicative senescence in superficial TCC, and inhibits the absolute migration capability in invasive bladder carcinoma cells
Chiung-Chi Penga, b, 1, Kuan-Chou Chena, c, 1, Robert Y. Penga, d, Charng-Cherng Chyaud, Ching-Hua Sua, e and Hsiu Mei Hsieh-Lia, f, , ,
aGraduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, 250 Wu-Xing Street, Taipei 110, Taiwan
bDepartment of Nursing, Cardinal Tien College of Nursing, No. 364, Chung Cheng Road, Hsintien City, Taipei Hsien 23148, Taiwan
cDepartment of Urology, Taipei Medical University Hospital, Taipei Medical University, 250 Wu-Xing Street, Taipei 110, Taiwan
dResearch Institute of Medicinal Science and Technology, Hung-Kuang University, No. 34, Chun-Chie Road, Shalu City, Taichung Hsien 43302, Taiwan
eGraduate Institute of Biomedical Material, Taipei Medical University, No. 250, Wu-Xing Street, Taipei 110, Taiwan
fDepartment of Life Science, National Taiwan Normal University, 88, Sec. 4, Ting-Chou Road, Taipei 116, Taiwan
Received 9 January 2006; revised 28 June 2006; accepted 6 July 2006. Available online 11 July 2006.
The Antrodia camphorata crude extract (ACCE), an extract obtained from a precious traditional Chinese folkloric herbal medicine Zhan-Ku (a camphor tree mushroom) since the 18th century, has showed rather significant inhibitory effects on the growth and proliferation of the transitional cell carcinomas (TCC) cell lines RT4, TSGH-8301, and T24.
On treatment with ACCE at 100 ìg/mL, the p53-independent overexpression of p21 with simultaneous down alteration of pRb was observed in RT4, which was thus speculative of proceeding through a mechanism of replicative senescence.
On the contrary treatment with ACCE, at 50 ìg/mL, resulting in simultaneous down-regulations of Cdc2 and Cyclin B1, with suppression of the absolute migrating capability of the two cell lines TSGH-8301 and T24, and eventually the cell deaths.
We conclude that ACCE can be rather effective and beneficial in suppression of both the superficial cancer cell line RT4 and the metastatic cell lines (TSGH-8301 and T24) through different mechanisms.
From the paper (Conclusion):
In conclusion, ACCE has showed rather different significant inhibitory effects on the growth and proliferation of TCC cell lines, RT4, TSGH-8301, and T24. In terms of the cell cycle regulatory protein expressions, RT4 proceeds most probably through the mechanism of replicative senescence, as evidenced by the p53-independent overexpression of p21 with simultaneous down alteration of pRb.
On the contrary, growth inhibition of TSGH-8301 and T24 as affected by simultaneous down-regulations of Cdc2 and Cyclin B1 were attributed to the insufficient and destabilized Cdc2Cyclin B1 complex formation. ACCE was shown to have effectively suppressed the most invasive T24 in terms of migration capability, an indicator of metastasis in vivo.
Moreover, ACCE is also effective for the superficial TCC cell line RT4.
Journal of Ethnopharmacology
Volume 109, Issue 1 , 3 January 2007, Pages 93-103