Resveratrol Supresses Growth and Induces Apoptosis in Bladder and Prostate Cancer Cell Lines I-Ling Lee, Jason R. Gee, H. B. Grossman, Anita L. Sabichi. U. T. M. D. Anderson Cancer Center, Houston, TX. Resveratrol is a polyphenolic phytoalexin abundant in a variety of plant foods. We have previously demonstrated inhibition of tumor growth by resveratrol in an animal model of bladder carcinogenesis. This study examined the effects of resveratrol in bladder and prostate cancer cell lines. Three human transitional cell carcinoma cell lines (UM-UC-1, UM-UC-3 and UM-UC-6) and three human prostate cancer cell lines (LNCaP, DU145, and PC3) were treated with resveratrol for 24 and 48 hours. Cell growth was measured by crystal violet assay. Cell cycle was measured using flow cytometry and the APO-BRDU kit was used to assess apoptosis. Cellular morphology was recorded by digital microscopy. Resveratrol suppressed growth in the bladder and prostate cell lines in a dose-dependent fashion with an IC50 between 50 and 100ėM. This inhibition was associated with an increase in the G0-G1 population in 5 of the 6 cell lines ranging from 24% to 52%. Treatment with 200ėM resveratrol for 48 hours resulted in significant apoptosis in UM-UC-1 (17%), UM-UC-3 (45%), UM-UC-6 (54%), LNCaP (43%), and DU145 (28%). Apoptosis was observed as early as 24 hours only in LNCaP (16%). No apoptosis was observed for the PC-3 cell line at either 24 or 48 hours. Altered cellular morphology with flattening of the cells was observed in all 6 cell lines following treatment with resveratrol for 48 hours at the IC50 concentrations. In conclusion, we found that resveratrol suppresses the growth of multiple human bladder and prostate cancer cell lines in a dose dependent fashion, with an IC50 between 50 and 100ėM associated with a G0–G1 cell cycle arrest. Apoptosis was induced in all the cell lines at 200ėM with the exception of the hormonally independent prostate cell line PC-3. Although apoptosis was not observed in PC-3, cell cycle arrest and possibly other factors may play a part in resveratrol–induced growth inhibition in PC-3 and the other cell lines. These findings support the investigation of resveratrol as a potential chemopreventive agent in bladder and prostate cancers. AACR Abstract Number: 4776 2003 Remember we are NOT Doctors and have NO medical training. This site is like an Encylopedia - there are many pages, many links on many topics. Support our work with any size DONATION - see left side of any page - for how to donate. You can help raise awareness of CAM.