Resveratrol increases BRCA1 and BRCA2 mRNA expression in breast tumour cell lines P Fustier1, L Le Corre1, N Chalabi1, C Vissac-Sabatier1, Y Communal2, Y-J Bignon1 and D J Bernard-Gallon1 1Laboratoire d'Oncologie Moléculaire, Centre Jean Perrin, 58 Rue Montalembert, BP 392, 63011 Clermont-Ferrand, France 2Laboratoire d'Immunologie, Centre Jean Perrin, 58 Rue Montalembert, BP 392, 63011 Clermont-Ferrand, France Correspondence to: Dr Y-J Bignon, Laboratoire d'Oncologie Moléculaire, Centre Jean Perrin, UMR 484 INSERM-UdA, 58 Rue Montalembert, BP 392, 63011 Clermont-Ferrand Cedex 1, France. E-mail: Yves-Jean.Bignon@cjp.u-clermont1.fr Abstract The phytochemical resveratrol, found in grapes, berries and peanuts, has been found to possess cancer chemopreventive effects by inhibiting diverse cellular events associated with tumour initiation, promotion and progression. Resveratrol is also a phyto-oestrogen, binds to and activates oestrogen receptors that regulate the transcription of oestrogen-responsive target genes such as the breast cancer susceptibility genes BRCA1 and BRCA2. We investigated the effects of resveratrol on BRCA1 and BRCA2 expression in human breast cancer cell lines (MCF7, HBL 100 and MDA-MB 231) using quantitative real-time RT-PCR, and by perfusion chromatography of the proteins. All cell lines were treated with 30 M resveratrol. The expressions of BRCA1 and BRCA2 mRNAs were increased although no change in the expression of the proteins were found. These data indicate that resveratrol at 30 M can increase expression of genes involved in the aggressiveness of human breast tumour cell lines. British Journal of Cancer (2003) 89, 168-172. Remember we are NOT Doctors and have NO medical training. This site is like an Encylopedia - there are many pages, many links on many topics. Support our work with any size DONATION - see left side of any page - for how to donate. You can help raise awareness of CAM.