Abstract Number: 5675 The anticarcinogenic effects of red wine polyphenols in a mouse skin model George Soleas, C. Linda Grass, P. David Josephy, Eleftherios P. Diamandis, David M. Goldberg, Liquor Control Board of Ontario, Toronto, ON, Canada; Pathology and Laboratory Medicine, Mt Sinai Hospital, Toronto, ON, Canada; Department of Chemistry & Biochemistry, University of Guelph, Guelph, ON, Canada; Pathology and Laboratory Medicine, Mt. Sinai Hospital, Toronto, ON, Canada; Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, ON, Canada. There has been growing interest in the analysis of certain polyphenols in wine, especially flavonoids, trihydroxystilbenes and phenolic acids, stimulated by intense research into their potential benefits to human health. One of their main properties in this regard is their antioxidant characteristic, which may contribute to prevention of atherosclerosis, inflammatory diseases, and cancer. A two stage CD-1 mouse skin cancer model using 9,10-dimethyl-1,2-benzanthracene (DMBA) as initiator and phorbol 12-myristate 13-acetate (TPA) as promoter was employed to compare the anti-tumorigenic activities of one polyphenol from each of four different classes: flavanols ((+)-catechin), stilbenes (trans-resveratrol), flavonols (quercetin) and hydroxybenzoic acids (gallic acid). Animals were treated with specific polyphenols at doses ranging from 0 to 25 ėmoles (dissolved in 200 ėL acetone), twice a week for eighteen weeks. The solution was applied topically to the shaved dorsal region of each animal. The relative potencies of the polyphenols were compared by evaluating the percentage inhibition of tumor formation (number of tumors in individual mice) and the number of mice developing one or more tumors. Probit analysis revealed that quercetin was the most (EC50 <1 ėmole) and gallic acid the least effective (EC50 5-10 ėmoles). (+)-Catechin and trans-resveratrol were intermediate, with EC50 values of 5 and 6 ėmoles, respectively. We have shown recently that trans-resveratrol is absorbed much more efficiently than (+)-catechin and quercetin in humans after oral consumption. Taking this and the relative concentrations in red wine into account, together with the present results, we suggest that trans-resveratrol may be the most effective anticancer polyphenol present in red wine as consumed orally by healthy human subjects Remember we are NOT Doctors and have NO medical training. This site is like an Encylopedia - there are many pages, many links on many topics. Support our work with any size DONATION - see left side of any page - for how to donate. You can help raise awareness of CAM.