Abstract Number: 5683 An evaluation of the total peroxyl radical scavenging capacities of various antioxidants utilizing the novel TOSC assay. Stephen W. Standage, Brad Green, Damon Cox, Byron K. Murray, Kim L. O'Neill, University of Rochester School of Medicine, Rochester, NY; Brigham Young University, Provo, UT. The Total Oxyradical Scavenging Capacity (TOSC) assay is a novel method for rapidly measuring the protective antioxidant abilities of pure antioxidant solutions. Briefly, peroxyl radicals, generated by the thermal homolysis of 2,2'-azobis-amidinopropane, oxidize alpha-keto-gama-methiolbutyric acid (KMBA) to produce ethylene. This free radical generation of ethylene is quantified by gas chromatographic analysis of reaction vial headspace. Ethylene production is reduced in the presence of antioxidants because peroxyl radical scavenging by those compounds inhibits KMBA oxidation. The amount of decrease in ethylene production compared to a control reaction without antioxidant provides a relative measure of antioxidant capacity. Herein we report the antioxidant activities of vitamin C, vitamin E, Grape Seed Extract (GSE), and Epigallocatechin-3-Gallate (EGCG) as measured by the TOSC assay. These four compounds were compared to the water-soluble vitamin E analogue Trolox. GSE was found to be the most active compound, demonstrating a peroxyl radical scavenging capacity 3.3 times greater than that of Trolox. GSE antioxidant activity was followed by that of EGCG, then vitamin C, and finally vitamin E. These results give strong indications that increased dietary or supplemental intake of these four substances can prove beneficial to combat biological oxidative damage mediated by free radical attack. Remember we are NOT Doctors and have NO medical training. This site is like an Encylopedia - there are many pages, many links on many topics. Support our work with any size DONATION - see left side of any page - for how to donate. You can help raise awareness of CAM.