Antioxidant and radical scavenging properties in vitro of polyphenolic extracts from red wine M. Lodovici (1), F. Guglielmi (1), C. Casalini (1), M. Meoni (1), V. Cheynier (2), P. Dolara (1) (1) Department of Pharmacology, University of Florence, Viale Pieraccini 6, 50139 Florence, Italy (2) Research Unit Biopolymers and Aromas INRA-ISVV, Place Viala 2, 34060 Montpellier Cedex, France Fax: +39-055/4271280 E-Mail: maura@pharm.unifi.it Summary Background & Aims Red wine polyphenols inhibit chemically-induced oxidative DNA damage in vivo in experimental animals through a mechanism which is still unclear. On this basis, we tried to clarify the mechanisms of inhibition of DNA oxidation in vitro by wine extracts containing monomeric and polymeric phenols (WE) and monomer-free complex polyphenols and tannins (WCPT) from red wine. Methods Oxidative DNA damage was induced by incubating DNA with GSH/Fe3+ or cumene hydroperoxide (CumOOH) in vitro and using 8-OH-2-deoxyguanosine (8-OHdG) levels as a measure of DNA oxidation. Levels of 8-OHdG were determined by HPLC coupled with electrochemical detector (ESA). Results and conclusions WCPT and WE, at µM concentrations, reduced concentration-dependently oxidative DNA damage induced by GSH/Fe3+. WCPT and WE also reduced DNA oxidation by CumOOH. In conclusion, complex polyphenols and tannin extracts from red wine, with or without small molecular phenols, prevent oxidative DNA damage through a dual mechanism, iron binding and direct free radical scavenging. European Journal of Nutrition Abstract Volume 40 Issue 2 (2001) pp 74-77 Int J Food Sci & Nutr Remember we are NOT Doctors and have NO medical training. This site is like an Encylopedia - there are many pages, many links on many topics. Support our work with any size DONATION - see left side of any page - for how to donate. You can help raise awareness of CAM.