Suppression of N-nitroso-N-methylurea-induced second generation rat mammary tumors with combined melatonin-tamoxifen therapy Lalita Kothari, Ami Kothan , Anita Borges, Indranil Mitra, Sharayu Kabre Cell Biology Division , Cancer Research Institute. Department of Pathology , Tumor Marker Laboratory , Tata Memorial Hospital, Tata Memorial Centre, Parel, Bombay 400012 Abstract Sprague-Dawley rats were injected with N-nitroso-N-methylurea (NMU) (50mg/kg b.wt.) and the primary tumor which developed within three months were excised. These tumors were found to be ER +ve (50±4.3 fmol/mg protein). Animals were regrouped following excision and melatonin (Mel) and/or tamoxifen (Tam) treatment was initiated immediately. There was uniform and significant delay in secondary tumor appearance in all the treated groups as compared to the untreated NMU-administered animals. The most effective suppression was observed in animals treated with combined therapy and histopathologically, these tumors were benign. The combination of melatonin and tamoxifen in women having ER+ve tumors appears to be a useful therapeutic approach. Neuroendocrinology Letters Vol. 18 No. 1 Remember we are NOT Doctors and have NO medical training. This site is like an Encylopedia - there are many pages, many links on many topics. Support our work with any size DONATION - see left side of any page - for how to donate. You can help raise awareness of CAM.